Flp-in cho
WebSeveral Flp-In™ host cell lines which stably express the lacZ-Zeocin™ fusion gene and contain a single integrated FRT site are available (see page 7 for ordering information). To express your gene of interest in 293, CV-1, CHO, 3T3, BHK, or Jurkat cells, you may want to use one of the Flp-In™ host cell lines to establish WebLike Cre, FLP is a tyrosine recombinase, but it originates from the yeast Saccharomyces cerevisiae, rather than from bacteriophage. In FLP-FRT, FLP is analogous to cre and FRT to loxP – FLP recognizes the FRT sites, and catalyzes recombination between them.
Flp-in cho
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WebOct 1, 2024 · Objective To construct a double transfected Flp-InTM CHO cell line stably expressing both cytochrome P450 family 2 subfamily A member 13(CYP2A13) and … WebFlp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain ... The Flp-In …
WebNov 11, 2013 · Flip-In CHO cells (FCHO) stably transfected by plasmid DNA were stained by PE-conjugated mouse-anti-human kappa chain antibodies (PE-Kappa) and then analyzed by FACS. ... Invitrogen (2006) Flp-In system for generating stable mammalian expression cell lines by Flp recombinase-mediated integration. Instruction Manual. Carlsbad, CA. 22. WebAug 8, 2016 · MIL75-pFRT-FTMK and POG44 were co-transfected (1:9) into Flp-In-CHO cells. After 48 h of transfection, the cells were cultured under selection pressure by hygromycin B (1 mg/ml).
WebAug 24, 2024 · Stable Flp-In™ CHO expression cell lines were generated by co-transfection of Flp-In™ expression construct (pcDNA™5/FRT/TO_FSHR) and the pOG44 plasmid (Invitrogen #V6005-20). Co-transfection was done using lipid-mediated transfection (Lipofectamine; Invitrogen #11668, Thermo Fisher Scientific Inc., Waltham, MA, USA … WebOct 1, 2024 · Objective To construct a double transfected Flp-InTM CHO cell line stably expressing both cytochrome P450 family 2 subfamily A member 13(CYP2A13) and multidrug resistance-associated protein 2(MRP2).
WebInvitrogen™ Flp-In™-CHO Cell Line. Designed for rapid generation of stable cell lines that express protein of interest from Flp-In expression vector. Includes: 3 x 10 6 cells are …
WebObjective To construct a double transfected Flp-In TM CHO cell line stably expressing both cytochrome P450 family 2 subfamily A member 13(CYP2A13) and multidrug resistance … portrush murder victim nameWebAll of the Flp-In ™ cell lines (except Flp-In -CHO; see the following section) contain a single integrated FRT site and stably express the lacZ-Zeocin™ fusion gene from the pFRT/lacZeo plasmid under the control of the SV40 early promoter (see the following diagram). The location of the FRT site in each optspt.me/cleanWebJan 13, 2024 · HEK & CHO mammalian cell lines in comparison. CHO mammalian cell lines have, similar to HEK cells, a long history of being used for the production of various … optsol consult and services limitedWebFlp-In™Host Cell Lines For your convenience, Invitrogen has available several mammalian Flp-In™host cell lines that stably express the lacZ-Zeocin™fusion gene from pFRT/lacZeo or pFRT/lacZeo2. Each cell line contains a single integrated FRT site as confirmed by Southern blot analysis. portrush locationportrush long range weather forecastWebDec 1, 2024 · CHO ori cell line was isolated and immortalized by Theodore Puck in 1956 [7]. Subclones were then further developed by introducing mutations or via adaptation to … optsoft incWebFlp-In™-293 Human embryonic kidney 1 × 107 cells, frozen R750–07 Flp-In™-CV-1 African Green Monkey kidney 1 × 107 cells, frozen R752–07 Flp-In™-CHO Chinese Hamster … optsol 150ct