WebpLVX-IRES-Puro Sequence and Map pLVX-IRES-Puro Lentiviral vector for bicistronic expression of a gene together with a puromycin resistance marker. Sequence Author: Clontech (TaKaRa) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7k Plasmids: Viral Expression & Packaging … WebNov 23, 2024 · A The efficiency of HBLV-m-PDPK1-3xflag-mCherry-PURO detected by RT-qPCR ( n = 3). B Immunoblot of cell lysates. Quantification of the ratio of PDPK1/β-actin ( …
Downregulating PDPK1 and taking phillyrin as PDPK1-targeting …
WebDec 3, 2024 · To construct short hairpin RNAs (shRNAs) targeting circ-TNPO3 or SNAIL, the shRNA sequences targeting the back-splicing junction of circ-TNPO3 were ligated into the HBLV-GFP-Puro lentiviral vector (Han Biomedical, Shanghai, China), and the shRNA sequences targeting mRNA of SNAIL were ligated into the HBLV-mcherry-BSD lentiviral … WebMammalian Expression, Lentiviral Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) NEB Stable Copy number High Copy Gene/Insert Gene/Insert name mCherry Species Synthetic Insert Size (bp) 711 Mutation no Cloning Information Cloning method Restriction Enzyme bristol county ma recorder of deeds
pLVX-mCherry-N1 Vector Information - takarabio.com
WebpLV-mCherry (Plasmid #36084) Print Enlarge View all sequences Purpose (Empty Backbone) 3rd generation lentiviral plasmid expressing mCherry Depositing Lab Pantelis Tsoulfas Publication Tsoulfas Lab plasmids … WebNov 23, 2024 · Alcoholic steatohepatitis (ASH) is a critical stage leading to cirrhosis and end-stage liver disease. However, its pathogenesis is still far from clearly understood, and a treatment that is widely... WebpCDH-EF1-MCS-T2A-Puro EF1 pCDH-EF1-MCS-T2A-copGFP EF1 Medium robust in most cell types, including primary differentiated cells pCDH-MCS-T2A-Puro-MSCV MSCV pCDH-MCS-T2A-copGFP-MSCV MSCV High hematopoietic / stem cell lines Single Promoter pCDH-CMV-MCS CMV pCDH-CMV-MCSr CMV pCDH-CMV-MCS-EF1-Puro CMV Dual … bristol county ma election results