Web21. feb 2024 · TEM analysis of SpRecA and EcRecA polymerisation on ssDNA.From a to h, representative electron micrographs images of ΦX ssDNA alone (A) or incubated with SpRecA and ATP (B), SpRecA, ATP and an ATP regenerating system (C), SpRecA and ATPγS (D), SpRecA and ATP-BeF 3 (E), with EcRecA and ATP (F), RecA Ec, ATP and an ATP … http://phenix.lbl.gov/pipermail/phenixbb/2024-August/024764.html
A national facility for biological cryo-electron microscopy
WebAs more protein structure models have been determined from cryogenic electron microscopy (cryo-EM) density maps, establishing how to evaluate the model accuracy and how to correct models in cases where they contain errors is becoming crucial to ensure the quality of the structural models deposited in the public database, the PDB. Web23. sep 2024 · phenix.resolve_cryo_em emd_20026_half_map_1_box.ccp4 emd_20026_half_map_2_box.ccp4 resolution = 2.2 dm_resolution = 1.8 seq_file = seq.dat … pfg saint genis laval
Introduction to Cryo EM - Thermo Fisher Scientific - US
WebPrevious message: [phenixbb] phenix.resolve_cryo_em Next message: [phenixbb] MicroED Imaging Center Messages sorted by: Hi Tom, Thanks for the help. I fixed the problem by assigning model_1= to a structure that was refined into the original cryo-EM map. The nightly build (Aug 24th dev-3965) does not read the sequence file. ... Web21. mar 2024 · G protein-coupled receptors (GPCRs) activate heterotrimeric G proteins by stimulating the exchange of guanine nucleotide in the Gα subunit. To visualize this mechanism, we developed a time-resolved cryo-EM approach that examines the progression of ensembles of pre-steady-state intermediates of a GPCR-G protein complex. Using … Web30. dec 2024 · A high-resolution electron cryo-microscopy structure of the mitochondrial respiratory supercomplex I + III2 from Arabidopsis thaliana shows that the component complexes stabilize each other. A ... pfhxs list